Investigation of the presence of G۳۵۴A (Cys۸۷Tyr) mutation in osteoprotegerin gene in women with osteoporosis inChaharmahal and Bakhtiari province

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_SKUMS-21-6_001

تاریخ نمایه سازی: 28 آذر 1400

Abstract:

Background and aims: Osteoprotegerin (OPG) is a competitive inhibitor of the differentiation and activity of osteoclasts, which inhibits thefinal stages of osteoclast formation and induces its apoptosis. In addition, OPG is considered as one of the most important candidate genesin the pathogenesis of bone diseases such as osteoporosis and idiopathic hyperphosphatasia. The G۳۵۴A (Cys۸۷Tyr) mutation in the OPGgene leads to idiopathic hyperphosphatasia. This mutation is probably related to osteoporosis. The purpose of this study was to investigatethe presence of G۳۵۴A (Cys۸۷Tyr) in women with osteoporosis in Chaharmahal and Bakhtiari province.Methods: In this descriptive-analytical study, the bone mineral density (BMD) of the femoral neck and lumbar spine of women referring toShahrekord bone densitometry centers was measured by the X-ray absorptiometry technique in ۲۰۱۳-۲۰۱۴. Based on T-scores, people withosteoporosis were identified and ۷۰ patients were enrolled in the study after receiving their consent. Finally, DNA was extracted from bloodsamples, amplified by polymerase chain reaction (PCR) technique, and sequenced by DNA sequencing method.Results: After DNA extraction from the blood, the quality and quantity were determined by gel electrophoresis and spectrophotometry,respectively. Then, the gene was amplified by the PCR method and the product was detected by gel electrophoresis, followed by sequencingthe samples to investigate the presence of the mutation. Eventually, genotypes associated with Cys۸۷Tyr mutation were not observed inthe studied population.Conclusion: In the present study, the G۳۵۴A (Cys۸۷Tyr) mutation associated with idiopathic hyperphosphatasia was not found in womenwith osteoporosis.

Authors

Seyedeh Zahra Mousavi

Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran

Morteza Dehghan

Department of Orthopedics, Shahrekord University of Medical Sciences, Shahrekord, Iran

Razieh Pourahmad

Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran