Evaluation of citrate consumption by microorganisms in anticoagulant bags of sodium citrate in case of contamination

Stability in the amount of sodium citrate in anticoagulant bags is very important. Ensuring that this substance is not reduced or degraded in all manufacturing processes up to storage is a requirement of pharmaceutical companies producing anticoagulants containing sodium citrate. Many citrate-consuming microorganisms can reduce the amount of sodium citrate present in the product, which doubles the importance of checking for the absence of these microorganisms before final sterilization. In the present study, Sodium citrate ۴% (w/v) bags were inoculated with half McFarland suspension of ۴ bacterial strains Pseudomonas aeruginosa, Serratia marcescens, Klebsiella Pneumoniae, Enterococcus Faecalis separately and incubated at ۳۵ ° C for ۱۲۰ hours until citrate was consumed. Citrate levels were measured at the pre-inoculation stage and then up to ۱۲۰ hours at regular intervals. The results showed that the amount of citrate remaining in the sample inoculated with K. Pneumoniae was significantly (p< ۰.۰۵) the lowest and in the samples inoculated with P. aeruginosa and E. Faecalis was significantly (p< ۰.۰۵) the highest.

Keywords:

anticoagulants , Blood , Citrate consuming bacteria , Plasma , Potentiometer , Sodium citrate