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Title

۱۷α-Hydroxy-۴-pregenen-۳-one (۱۷ αP) assay, using acetylcholinesterase enzyme as tracer

Year: 1384
COI: JR_JIFRO-5-1_002
Language: EnglishView: 78
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Abstract:

۱۷a-hydroxy-۴-pregnen-۳-one (۱۷aP) hormone is a precursor of other steroid hormones and radioimmunoassay has already been used to measure it, but a simple and rapid "Enzyme Linked Immunosorbant Assay" (ELISA) is described and validated here. A general procedure for prepara-tion of the acetylcholinesterase labelled steroid is described, which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only ۱۰ ml of plasma. The ELISA was applied to measure ۱۷, ۲۰a-hydroxy-۴-pregnen-۳-one (۱۷, ۲۰aP) steroid production from ۱۷aP by ۲۰ a-hydroxy steroid dehydrogenase (۲۰a-HSD) from sperm of roach (Rutilus rutilus). The results showed that cyprinid sperm contains potent and active ۲۰a-HSD enzymes which produce ۱۷, ۲۰aP hormone from ۱۷aP substrate. 

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This Paper COI Code is JR_JIFRO-5-1_002. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1366754/

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Ebrahimi, M.,1384,۱۷α-Hydroxy-۴-pregenen-۳-one (۱۷ αP) assay, using acetylcholinesterase enzyme as tracer,https://civilica.com/doc/1366754

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