Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe۳O۴ nanoparticles

Purpose: Auraptene (AUR) is a monoterpene coumarin compound with several biological activities specifically anti-cancer. The bioavailability of AUR in biological fluids is negligible, thus, the cytotoxicity of this compound for the target cells is low. Herein, the synthesis of AUR-coated Fe۳O۴ nanoparticles is presented as a strategy to increase the cytotoxicity of AUR on PC۳, DU۱۴۵, and LNCaP prostate cancer cells.Methods: Fe۳O۴ nanoparticles were synthesized via co-precipitation method, coated with AUR and stabilized by dextran. They were characterized by X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS) analysis, and vibrating sample magnetometry (VSM). In vitro release test for coated nanoparticles was performed in both physiologic (pH= ۷.۴) and acidic (pH= ۵.۵) environments. Cytotoxicity for prostate cancer cells was evaluated by AlamarBlue assay and the results were analyzed by one-way and two-way ANOVA tests.Results: Characterization outcomes represented the formation of magnetic nanoparticles with good crystalline structure, relatively spherical shape and superparamagnetic properties. AUR release profile from nanoparticles demonstrated that coated nanoparticles are able to inhibit burst release of this compound. AUR release was remarkably higher in acidic medium that can be advantageous for treating tumor regions. Cytotoxicity results indicated that AUR had a very low toxicity against prostate cancer cells at the tested concentrations. In contrast, AUR-coated Fe۳O۴ nanoparticles were significantly cytotoxic on all the cell lines.Conclusion: The coating of AUR on the surface of Fe۳O۴ nanoparticles was a successful approach to enhance the efficacy and cytotoxicity of this compound.

Keywords:

Auraptene , Coumarin , Cytotoxicity , Fe۳O۴ nanoparticles , Prostate cancer