Navid Dadashpour Davachi - Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
Reza Masoudi - Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Pawel Bartlewski - Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph. Guelph, Ontario, Canada
Bahareh Ahmadi - Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph. Guelph, Ontario, Canada
Massod Didarkhah - Faculty of Agriculture Sarayan, University of Birjand, Birjand, Iran

 BACKGROUND: Rabbits are induced ovulators with the ovulatory process being triggered by neuro-hormonal impulses gen-erated during natural mating. When applying artificial insemination (AI), an array of biostimulation techniques and/or exogenous hormones, such as gonadotropin-releasing hormone (GnRH) or its analogues must be used to induce ovulation. However, the effect of biostimulation techniques and exogenous hormones is not always satisfactory and the use of GnRH analogues is asso-ciated with high production costs. Therefore, the development of an alternative inexpensive, efficient, and safe treatment for ovulation induction in artificially inseminated does is urgently needed. OBJECTIVES: In the present study, we examined and compared the effects of mated doe serum (MDS) and GnRH analogue (Gonadorelin) administered immediately after AI on the circulating concentrations of luteinizing hormone (LH) and fertility in New Zealand does. METHODS: Forty artificially inseminated does were allocated to four equinumerous groups. The Control G, Treatment G, Control M, and Treatment M groups received ۰.۲ mL of saline intramuscular (IM), ۰.۸ μg of Gonadorelin dissolved in ۰.۲ mL of saline IM, ۲.۵ mL of mixed-sex normal rabbit serum intravenous (IV),and ۲.۵ mL of MDS/doe IV, respectively. RESULTS: A peak in systemic LH concentrations occurred earlier in Treatment M, compared to Treatment G does (۷۱ vs. ۱۰۷ min post-AI, respectively; P≤۰.۰۵). Mean LH concentrations did not vary (P≤۰.۰۵) from the pre-AI values in neither of the control groups. Serum LH concentrations remained higher (P≤۰.۰۵) in the Treatment M group, in comparison with Treatment G does during۳۰-۹۰ min post-AI. However, LH was higher (P≤۰.۰۵) in the Treatment G group than the Treatment M group ۱۲۰ and ۱۶۰ min post-AI. Gonadorelin and MDS injections both resulted in the same kindling rate of ۸۰% at each of the four con-secutive AIs (initiated ۳۰ days postpartum) and were significantly greater than that recorded in the control animals (۲۰%). CONCLUSIONS: It can be concluded that MDS administration is an effective treatment for inducing ovulation in rabbits with repeatability similar to that achieved with a GnRH analogue

GnRH, Induced ovulatory, Mated doe serum, Neuro-hormonal reflex, Rabbit