Elham Asa - Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
Rahil Janatifar - Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
Seyedeh Saeideh Sahraei - Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
Atefeh Verdi - Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
Naser Kalhor - Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.

Background: Failed oocyte activation following intracytoplasmic sperm injection (ICSI) as a result of calcium deficiency is a major challenge. Objective: We compared the effect of cult-active medium (CAM) on ICSI outcomes in obstructive azoospermia cases. Materials and Methods: The present study was conducted with ۱۵۲ ICSI cases, classified into CAM and control groups. The injected oocytes in the control group were cultured in the cleavage medium, while in the artificial oocyte activation group, oocytes were chemically activated through exposure to ۲۰۰ µL of CAM for ۱۵ min. Fertilization and cleavage rates, quality of embryos, and biochemical pregnancy and live birth rates were assessed in both groups. Results: There were significant differences between the groups in terms of fertilization and cleavage rates after using the CAM in the percutaneous epididymal sperm aspiration (PESA) subgroup (p = ۰.۰۵, p ≤ ۰.۰۰۱) and in the testicular sperm extraction subgroup (p = ۰.۰۲, p = ۰.۰۴), compared to their control groups. Also, the pregnancy rate was significantly higher in the PESA-CAM subgroup (p = ۰.۰۳). The PESA-CAM subgroup demonstrated a significant difference in embryo quality after ICSI (p = ۰.۰۴). Unsuccessful embryo transfer and abortion were lower in both subgroups compared to the control groups, but this difference was not significant. Surprisingly, live birth rate was higher in the PESA-CAM subgroup (p = ۰.۰۳). Conclusion: CAM treatment could improve fertilization and cleavage rates in obstructive azoospermia participants. It had a significant effect on embryo quality, and pregnancy and live birth rates in PESA cases.

Calcium ionophore, Obstructive azoospermia, Fertilization, ICSI., یونوفور کلسیم, آزواسپرمی انسدادی, لقاح, ICSI.