Xiao-Guang Chen - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou ۴۵۰۰۵۲, Henan Province, P.R. China
Bing-Hua Dou - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou ۴۵۰۰۵۲, Henan Province, P.R. China
Jin-Dou An - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou ۴۵۰۰۵۲, Henan Province, P.R. China
Song Feng - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou ۴۵۰۰۵۲, Henan Province, P.R. China
Na Liu - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou ۴۵۰۰۵۲, Henan Province, P.R. China
Guang-Yao Sheng - Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou ۴۵۰۰۵۲, Henan Province, P.R. China

Objective(s): MAGI۲-AS۳ is a cancer suppressor gene of multiple malignancies. Acute lymphoblastic leukemia (ALL) is an important type of leukemia that especially occurs in children. Our work evaluated the modulation of MAGI۲-AS۳ in ALL.Materials and Methods: qPCR and Western blotting were adopted for detection of target molecular expression. Growth and apoptosis were determined by CCK۸ assay and Annexin V/PI staining. Glycolysis was detected by commercial kits. The direct binding between miR-۴۵۲-۵p and MAGI۲-AS۳ or FOXN۳ was assessed by luciferase reporter assay. Tumor growth was measured in nude mice in vivo.Results: MAGI۲-AS۳ was down-regulated in ALL. Enforced expression of MAGI۲-AS۳ inhibited growth and glycolysis while promoting apoptosis of ALL cells. Moreover, MAGI۲-AS۳ up-regulated FOXN۳ via sponging miR-۴۵۲-۵p. FOXN۳ depletion abrogated MAGI۲-AS۳-mediated anti-cancer action. More importantly, MAGI۲-AS۳ repressed ALL cell growth in nude mice through regulation of miR-۴۵۲-۵p/FOXN۳. Conclusion: MAGI۲-AS۳ inhibits ALL development via modulating miR-۴۵۲-۵p/FOXN۳.

ALL, Apoptosis, FOXN۳, Glycolysis, Growth, MAGI۲-AS۳, miR-۴۵۲-۵p