C-Kit+ cells can modulate asthmatic condition via differentiation into pneumocyte-like cells and alteration of inflammatory responses via ERK/NF-ƙB pathway
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 25، Issue: 1
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 194
This Paper With 7 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-25-1_013
تاریخ نمایه سازی: 2 بهمن 1400
Abstract:
Objective(s): The exact role of the progenitor cell types in the dynamic healing of asthmatic lungs is lacking. This investigation was proposed to evaluate the effect of intratracheally administered rat bone marrow-derived c-kit+ cells on ovalbumin-induced sensitized male rats.Materials and Methods: Forty rats were randomly divided into ۴ groups; healthy rats received phosphate-buffered saline (PBS) (C); sensitized rats received PBS (S); PBS containing C-kitˉ cells (S+C-kit-); and PBS containing C-kit+ cells (S+C-kit+). After two weeks, circulatory CD۴+/CD۸+ T-cell counts and pulmonary ERK/NF-ƙB signaling pathway as well as the probability of cellular differentiation were assessed. Results: The results showed that transplanted C-Kit+ cells were engrafted into pulmonary tissue and differentiated into epithelial cells. C-Kit+ cells could increase the number of CD۴+ cells in comparison with the S group (P<۰.۰۰۱); however, they diminished the level of CD۸+ cells (P<۰.۰۱). Moreover, data demonstrated increased p-ERK/ERK ratio (P<۰.۰۰۱) and NF-ƙB level (P<۰.۰۵) in sensitized rats compared with the C group. The administration of C-kit+, but not C-Kit-, decreased p-ERK/ERK ratio and NF-ƙB level compared with those of the S group (P<۰.۰۵). Conclusion: The study revealed that C-Kit+ cells engrafted into pulmonary tissue reduced the NF-ƙB protein level and diminished p-ERK/ERK ratio, leading to suppression of inflammatory response in asthmatic lungs.
Keywords:
Authors
Fatemeh Mirershadi
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Mahdi Ahmadi
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Reza Rahbarghazi
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Hossain Heiran
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Rana Keyhanmanesh
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :