Evaluation of P۵۳ and CK۲۰ Immunohistochemical Markers in Comparison with Morphologic Findings in Low- and High-grade Urothelial Carcinomas

Background & Objective: Urothelial carcinoma is the seventh most common cancer in the world. The histological classification of papillary carcinoma is one of the most important determinants for its prognosis. Sometimes there is an overlap in the extent of the tumor, and the accurate microscopic diagnosis of the tumor is not always easy. The aim of this study was to evaluate P۵۳ and CK۲۰ immunohistochemical markers in comparison with morphologic findings in low- and high-grade urothelial carcinomas.Methods: For this descriptive study, urinary bladder samples were collected from ۵۰ cancer patients who had undergone biopsy and surgery in Shohaday-e Tajrish Hospital of Tehran, Iran, during the years ۲۰۱۵-۲۰۱۶. P۵۳ and CK۲۰ were studied, and the demographic and histopathological characteristics of the tumor were also analysed.Results: The mean age of patients enrolled in this study (۴۸ males and ۲ females) was ۶۵.۸±۱۱.۹. Twenty-five cases presented with low-grade and ۲۵ cases presented with high-grade papillary urothelial carcinomas. Sensitivity, specificity, and positive and negative predictive values for P۵۳ were ۴۸%, ۸۰%, ۷۰.۵%, and ۶۰.۶%, respectively, while the same values for CK۲۰ were ۴۴%, ۹۲%, ۸۴.۶%, and ۶۲.۲%, respectively. Immunohistochemical results were also positively correlated with the extent of the tumor. Conclusion: Based on the results, P۵۳ and CK۲۰ may serve as specific markers for diagnosis of low- and high-grade papillary urothelial carcinoma but not sensitive. P۵۳ and ck۲۰ staining have also a high specificity as ۸۰% and ۹۲% and low sensitivity compared to the low and high morphology of papillary carcinoma, thus their positive and their staining intensity are valuable for diagnosis, but their negative results are not determinant.

Keywords:

CK۲۰ , Grade , p۵۳ , Urothelial carcinoma