- - - Department of Mathematics and Physical Sciences, Maasai mara university, P.O Box ۸۶۱-۲۰۵۰۰, Narok, Kenya
- - - Department of Mathematics and Physical Sciences, Maasai mara university, P.O Box ۸۶۱-۲۰۵۰۰, Narok, Kenya
- - - Department of Mathematics and Physical Sciences, Maasai mara university, P.O Box ۸۶۱-۲۰۵۰۰, Narok, Kenya
- - - Department of Mathematics and Physical Sciences, Maasai mara university, P.O Box ۸۶۱-۲۰۵۰۰, Narok, Kenya
- - - Department of Chemistry, Multimedia university of Technology, P.O Box ۳۰۳۰۵-۰۰۱۰۰, Nairobi, Kenya
- - - The Centre for Innovation, New and Renewable Energy (CINRE) Department, Maasai mara university, P.O Box ۸۶۱-۲۰۵۰۰, Narok, Kenya
- - - Department of Mathematics and Physical Sciences, Maasai mara university, P.O Box ۸۶۱-۲۰۵۰۰, Narok, Kenya
- - - School of Physics, University of Witswatersrand, Private Bag ۳, ۲۰۵۰, South Africa
- - - Department of Chemistry, Multimedia university of Technology, P.O Box ۳۰۳۰۵-۰۰۱۰۰, Nairobi, Kenya

Purpose Application of Tithonia diversifolia leaves as organic fertilizer is common in most rural regions in Africa. There is usually time period for curing between preparation and application stage. However, this curing period leads to reduction in fertilizer efficiency. This study aimed at analyzing the change in functional groups, particle morphology, size and elemental composition from preparation and application stages of T. diversifolia leaves as organic fertilizer. Method The fertilizer was synthesized from T. diversifolia leaves. The synthesized fertilizer was stored at room temperature and pressure conditions for a grace period of ۵ days; as is norm. Characterization of the samples at the two stages was done by FT-IR (functional groups), SEM (morphology), TEM (particle size) and EDS (elemental composition). Results The results indicated reduction or complete loss of amine groups and carboxylic groups over this period. The fertilizer particles agglomerated over the storage period. Particle size increased from ۱۱۲ nm to ۱۳۳ nm diameter. There was a significant variation in the elemental composition between preparation and application time of the fertilizer samples. Conclusion These findings prompt for better storage techniques to be used or reduction in curing and storage period of the fertilizers to minimize loss of plant nutrients.

Tithonia diversifolia, Organic Fertilizer, Preparation, Application stage