Research Article: Effects of canola protein hydrolysate (CPH) on growth performance, blood biochemistry, immunity, and gastrointestinal microbiota of beluga (Huso huso) juveniles

This study assessed the effects of diets supplemented with canola protein hydrolysate (CPH) on growth performance, blood biochemistry, immunity, and gastrointestinal microbiota of beluga (Huso huso) juveniles. CPH concentrations of ۰, ۳۰۰, ۴۰۰, and ۵۰۰ mg kg-۱ diet were denoted as control, CPH۳۰۰, CPH۴۰۰, and CPH۵۰۰, respectively and were added to fish basal diet. ۸۴۰ beluga juveniles (۳۰±۶ g) were assigned into the four dietary treatments and fed for ۵۶ days. The diet supplemented with ۵۰۰ mg kg-۱ of CPH significantly decreased food conversion ratio (FCR). Condition factor (CF), specific growth rate (SGR), body weight gain (BWI), standard length (SL), total length (TL), survival rate, and food consumption demonstrated a CPH dose-dependent increase, with highest values in the fish treated with CPH۵۰۰. Concentrations of blood (serum) cholesterol, triglyceride, and glucose decreased in a CPH-concentration dependent manner as well. Red blood cells (RBC) and white blood cells (WBC) increased and decreased, respectively in all fish treated with CHP, especially in CPH۵۰۰ treatment. Moreover, gastrointestinal population of Lactobacillus spp. and total bacteria were significantly enhanced in the treated groups, with the highest loads of total bacterial (۲.۱×۱۰۶ CFU) and Lactobacillus spp. (۱.۶×۱۰۴ CFU) in CPH۵۰۰ treatment. Taken together, CPH could be introduced as an alternative for fishmeal in beluga diet, although more studies are needed to be conducted to elucidate the underlying mechanisms by which CPH improves fish growth.

Keywords:

Bacterial population , Canola , Growth performance , Hydrolyzed protein