S.E. Roohani Rankouhi - Department of Fishery, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
M. Mohammad Nejad - Department of Fisheries, Bandar Gaz Branch, Islamic Azad University, Bandar Gaz, Iran
H. Faghani Langrodi - Department of Fisheries, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
A. Aghaei Moghadam - Inland water Aquatic Resources Research Center, Gorgan, Iran

Kakuti, Ziziphora clinopodioides, is a medicinal herb found in Iran, but its potential benefits have not been studied on fish. Thus, the aim of the present study was to investigate the effects of Kakuti aqueous extract on the plasma antioxidant and enzymatic parameters in common carp, Cyprinus carpio. The aqueous extract was prepared by boiling Kakuti leaves for ۲h. Four diets containing %۰ (Ctrl), %۰.۵ (۰.۵E), %۱ (۱E), and %۲ (۲E) of Kakuti extract were fed to fish within a ۶۰-day period. For each diet, three tanks, each with ۱۵ fish with initial weight of ۴۶.۲±۰.۱۶g, were assigned. Then, blood samples were taken from all treatments to assess plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glucose, superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA). The results showed that %۱ Kakuti extract significantly decreased plasma AST and ALP, but increased CAT activities. There was no significant effect of dietary treatments on plasma ALT activity. ۰.۵ and %۱ Kakuti extract decreased plasma glucose levels significantly, but increased plasma SOD activity. ۱ and %۲ Kakuti extract decreased plasma MDA levels significantly. According to the results, Kakuti extract is capable of augmenting antioxidant strength, that in turn, may be the reason for boosted tissue health in common carp. Dietary %۱ Kakuti extract is recommended for inclusion in common carp diet.

Ziziphora clinopodioides, Common carp, Antioxidant, Immune system, Blood factors, Plasma enzymatic