Evaluation the performance of anaesthetic effects of Tobacco and Ketamine on Grass carp, Ctenopharyngodon idella (Valenciennes, ۱۸۴۴)

The present study aimed to determine the optimal concentration of three anaesthetics, name MS۲۲۲, ketamine, and tobacco extract, in grass carp juveniles (۱۰۷.۷۸±۲۲.۱۱ g body weight and ۲۲۶.۷۴±۱۳.۰۶ mm total body length). Treatments were as follows: ۱۰۰ mg L-۱ of MS۲۲۲ (control group), ۵.۵, ۸, ۱۱, ۱۸.۵, and ۲۲ mg L-۱ of ketamine, and ۱۰, ۱۳.۳۳, ۲۰, ۲۶.۲۷, and ۴۰ mg L-۱ of tobacco extract. In addition, changes in blood and biochemical parameters were measured at different concentrations. Considering the anaesthesia induction and recovery durations times, the optimal concentration for anaesthesia of studied substances was obtained as follows: ۱۰۰ mg L-۱ of MS۲۲۲ (۱۳۹.۰۰±۲۰.۰۰ seconds), ۱۸.۵ mg L-۱ of ketamine (۱۴۰.۱۰±۲۰.۲۰ seconds), and ۴۰ mg L-۱ of tobacco extract (۱۷۴.۰۰±۲۰.۰۹ seconds). Based on the results, the shortest recovery time from anaesthesia was observed at ۲۶.۲۷ mg L-۱ of tobacco extract (۸۷۰.۰۰±۵.۳ seconds) and ۸ mg L-۱ of ketamine (۹۶۷.۰۰±۵.۵ seconds), respectively. The results indicated that the anaesthesia induction and recovery duration was dependent on the concentration of anaesthetics. Moreover, significant differences were observed between the three anaesthetics in terms of blood parameters. The study findings also revealed a significant difference between treatments in biochemical parameters (cortisol, glucose, and lactate). 

Keywords:

Anaesthetics , Blood , Grass Carp , Ctenopharyngodon idella , MS۲۲۲ , Ketamine , Tobacco