Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis

PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond ۱ were gibel carp and grass carp, and the combined density was ۴۶۲۲۴ fingerling/ha (gibel carp/grass carp/silver carp/bighead carp, ۱۷:۴:۶:۱). The main culture species of pond ۲ was gibel carp, and the combined density was ۳۷۵۵۱ fingerling/ha (Gibel carp/silver carp/bighead carp, ۵۲:۱:۱). Water samples were collected monthly. The results showed that the annual average concentrations of TP and PO۴-P in pond ۱were significantly higher than pond ۲ (p>۰.۰۵). The concentration of chlorophyll a (chl a) has no significantly difference between pond ۱ and pond ۲. DGGE profiles of ۱۸S rRNA gene fragments from the two ponds revealed that the diversity of eukaryotic plankton assemblages was highly variable. ۹۱ bands and ۷۱ bands were detected in pond ۱ and pond ۲, respectively. The average Shannon–Wiener index of pond ۱ was significantly higher than pond ۲. Canonical correspondence analysis (CCA) revealed that temperature played a key role in the structure of the eukaryotic plankton community in both ponds, but the nutrient concentration did not affect it. Our results suggest that DGGE method is a cost-effective way to gain insight into seasonal dynamics of eukaryotic plankton communities in culture ponds, and the increase in the number of filter-feeding silver carp and bighead carp could increase the diversity of the eukaryotic plankton community.

Keywords:

۱۸S rRNA genes , PCR-denaturing gradient gel electrophoresis , CCA , Eukaryotic plankton community , Environmental factors