H. Rajabi Islami
N. Arab
R. Assare
M.I. Rastravan
R. Ebtekari

This study was aimed to evaluate the effect of L-ascorbyl-۲-polyphosphate as a dietary ascorbic acid source on blood parameters of Caspian brown trout (Salmo trutta caspius), including red blood cell (RBC) count, white blood cell (WBC) and WBC differential. A total number of ۶۰۰ Caspian brown trout (۹.۶±۰.۶ g) fingerlings were randomly distributed in triplicates among five treatments each containing ۴۰ specimens. Experimental diets were also prepared by adding ۰, ۵۰, ۱۰۰, ۲۰۰ and ۴۰۰ mg kg-۱ L-Ascorbyl-۲-Polyphosphate to the basal diet. Feeding was done for nine weeks in each treatment. The survival rate in all treatments was ۱۰۰%. The results showed a significant increase in RBC, hemoglobin, hematocrit, WBC and lymphocyte (p<۰.۰۵) by supplementing L-ascorbyl-۲-polyphosphate compared to the control treatment. The fish fed by ۲۰۰ mg ascorbic acid kg-۱ diet had the maximum hemoglobin and hematocrit in comparison with the other treatments. The current research showed that dietary L-ascorbyl-۲-polyphosphate influences the complete blood count of Caspian brown trout while fingerlings fed with the optimum amounts of ۲۰۰ mg ascorbic acid kg-۱ diet for a period of ۹ weeks trail.

Ascorbic acid, Growth, Hematological parameters, Salmo trutta caspius