Determination of plasma vitellogenin levels and localization of vitellogenin in liver of Lake Van pearl mullet (Chalcalburnus tarichi Pallas, ۱۸۱۱)

This study focused on hepatosomatic and gonadosomatic index and levels of plasma ۱۷β-estradiol (E۲), vitellogenin (Vtg) and total protein and determined the localization of Vtg in the liver during vitellogenesis in female Chalcalburnus tarichi, an endemic cyprinid species living in the Lake Van, Turkey. The levels of plasma E۲, Vtg and total protein in female fish increased during vitellogenesis and they are positively correlated with each other. According to the results from the female fish, the lowest levels of E۲ and Vtg were measured in December at ۱۲۰.۶۰±۲۳.۳۸ pg ml-۱ and ۱.۱۰±۰.۲۶ mg ml-۱, respectively. The highest levels of plasma E۲ and Vtg were in May (۸۳۳.۴۰±۲۱۱.۵۸ pg ml-۱) and April (۳۱.۳۱±۴.۹ mg ml-۱), respectively. The Vtg-positive hepatocytes were not uniform in liver of fishes. Generally, the hepatocytes surrounding the portal and central veins were strongly Vtg positive. These results showed that plasma Vtg levels in female fish increased during vitellogenesis related to plasma E۲ and gonadosomatic index.

Keywords:

Vitellogenesis , ۱۷β-estradiol , Gonadosomatic index , Chalcalburnus tarichi , Lake Van