Karyological analysis of Cyprinion macrostomum Heckel, ۱۸۴۳, from Godarkhosh River, Ilam Province, Iran

In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, ۱۸۴۳, was investigated through examining metaphase chromosomes of seven fish with mean weight ۳۰±۵g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as ۲n=۵۰. The karyotype consisted of ۵ metacentric, ۱۲ submetacentric and ۸ telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as ۰-۵۰, ۱-∞, and ۸۴, respectively.

Keywords:

Bigmouth lotak , Cyprinion macrostomum , Godarkhosh River , Iran , Karyotype.