Nanobiosensor designing with molecular framework polymer method compared with agent-linked nanosilica biosensor for Staphylococcus aureus exotoxin detection

Considering the ever increasing population and industrialization leading to developmental trend of humankind's life, we are hardly able to detect the toxins produced in food products using traditional techniques. In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, which are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenised connection between exotoxin amino acid and meta acrylic acid is made that would function as the selective absorption for that. Then in the second stage, based on the bacterial antibody connection to nanoparticle, a sensor was used. In this part of the research, as the basis for absorption for the recognition of bacterial toxin, medium sized silica nanoparticles of ۱۰ nanometer in the form of solid powder were utilized with Notrino brand. Then the suspension produced from agent-linked nanosilica which was connected to bacterial antibody was positioned near the samples of distilled water, that were contaminated with Staphylococcus aureus bacterial toxin with the density of ۱۰-۳, so that in case any toxin exists in the sample, a connection between toxin antigen and antibody would be formed. Finally, the light absorption related to the connection of antigen to the particle attached antibody was measured using spectrophotometers. The results indicate that the molecular framework polymer sensor is capable of detecting up to the density of ۱۰-۳, but not lower than that, whereas using the second sensor, up to ۱۰-۴ of density is detectable. Additionally, the sensitivity of the sensors were examined after ۶۰ days and the first sensor by the day of ۲۸ and the second sensor by the ۵۶ day had confirmatory results and started to decrease after those time periods

Keywords:

Nanobiosensor , Staphylococcus aureus , Exotoxin , Molecular Framework Polymer Nanosensor.