Lavender protects H۹c۲ cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK۲/STAT۳ pathway

Objective(s): This study was conducted to examine the therapeutic effects of lavender oil (LO) against oxygen-glucose deprivation (OGD)-induced injury in vitro model.Materials and Methods: In this study, the OGD model was induced in the H۹C۲ cell line, and then the cells were treated with LO (۱۰, ۱۰۰, ۱۰۰۰, and ۱۰۰۰۰ μg/ml). The anti-inflammatory activity of LO (Jak۲/Stat۳) was evaluated by immunocytochemical assay. Likewise, the p-ERK/ERK level was measured by western blotting.Results: Compared with only the OGD-induced injury model, cell survival increased after treatment with LO. Our results showed that ۱۰۰ μg/ml of LO significantly decreased the expression of Jak۲/Stat۳ and the apoptotic activity ۷۲ hr after reperfusion compared with the control group. Likewise, significant increases were observed in p-ERK/ERK in LO-treated groups. Conclusion: Collectively, these findings confirm that LO can be a good candidate to reduce OGD-induced injury in the H۹C۲ cell line through targeting Jak۲/Stat۳ and ERK pathways.

Keywords:

H۹C۲ cells , Inflammation , Ischemia/Reperfusion injury , Jak۲ / Stat۳ , Lavender oil , p-ERK / ERK