Shaghayegh Askarian-Amiri - Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, ۷۱ Avenue des Martyrs, ۳۸۰۰۰ Grenoble, France
Hoda Fotovat eskandari - Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
Fatemeh Ramezani - Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
Gelare Vahabzadeh - Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Nahid Aboutaleb - Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran

Objective(s): This study was conducted to examine the therapeutic effects of lavender oil (LO) against oxygen-glucose deprivation (OGD)-induced injury in vitro model.Materials and Methods: In this study, the OGD model was induced in the H۹C۲ cell line, and then the cells were treated with LO (۱۰, ۱۰۰, ۱۰۰۰, and ۱۰۰۰۰ μg/ml). The anti-inflammatory activity of LO (Jak۲/Stat۳) was evaluated by immunocytochemical assay. Likewise, the p-ERK/ERK level was measured by western blotting.Results: Compared with only the OGD-induced injury model, cell survival increased after treatment with LO. Our results showed that ۱۰۰ μg/ml of LO significantly decreased the expression of Jak۲/Stat۳ and the apoptotic activity ۷۲ hr after reperfusion compared with the control group. Likewise, significant increases were observed in p-ERK/ERK in LO-treated groups. Conclusion: Collectively, these findings confirm that LO can be a good candidate to reduce OGD-induced injury in the H۹C۲ cell line through targeting Jak۲/Stat۳ and ERK pathways.

H۹C۲ cells, Inflammation, Ischemia/Reperfusion injury, Jak۲ / Stat۳, Lavender oil, p-ERK / ERK