In vitro effect of Nanosilver toxicity on fibroblast and mesenchymal stem cell lines

 Nanotechnology presents countless opportunities to develop new and improved consumer products for the benefit of the society . A most prominent nanoproduct is nanosilver. Nanosilver particles are generally smaller than ۱۰۰ nm and contain ۲۰–۱۵,۰۰۰ silver atoms. Despite the wide application of nanomaterials, there is a serious lack of information concerning their impact on human health. In the previous study we reported the cytotoxic of nanosilver on osteoblast G۲۹۲ cancer cell line and the amount of IC۵۰ determined as ۳.۴۲ µg/ml (Moaddab et al., Iran. Nano Lett., Vol. ۱, No. ۱, January ۲۰۱۱, pp. ۱۱-۱۶). The purpose of the present study is to assess the biological assay of nanosilver on two normal cell lines of fibroblast (HF۲), and mesenchymal stem cells . The effect of nanosilver on these cells is evaluated by light microscopy, and by cell proliferation and standard cytotoxicity assays. The results demonstrate a concentration-dependent toxicity for the cells tested, and IC۵۰ was determined as ۶.۳۳, and ۶.۶۸ µg/ml in mesenchymal stem cell, and fibroblast HF۲, respectively. There is no significant difference between the ۲۴ h and ۴۸ h of cells exposure to nanosilver. The results show that Nano-Ag possesses low toxicity to normal cells and can display potential application in cancer chemoprevention and chemotherapy.

Keywords:

Nanosilver , toxicity test , mesenchymal stem cell , fibroblast (HF۲)