Quantitive characteristics and chemical composition in Caspian Roach (Rutilus rutilus caspicus) sperm

In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites of seminal plasma (glucose, cholesterol and protein) were measured. The Na+ and K+ ions correlated negatively with spermatozoa motility (r = -۰.۰۵۱۸, p<.۰۵ and r =-۰.۳۵۹۷, p<.۰۱) respectively. Also, there were significant positive correlations between Ca۲+ and Cl- ions with spermatozoa motility (r = ۰.۲۹۴۵, p<.۰۵ and r= ۰.۱۳۷۹, p<.۰۱), respectively. Mg+۲ was positively correlated with glucose and protein (r = ۰.۰۴۶, p<.۰۵ and r = ۰.۶۹۴, p<.۰۵), respectively. On the other hand, a significant positive relationship was found between Na+ and K+ (r = ۰.۵۴۸, p<.۰۱). These parameters can be used to evaluation of sperm quality and collecting information about developing procedures for artificial fertilization of roach.

Keywords:

Sperm motility , Seminal plasma , Sperm quality , Roach