Analysis of saline activator solution effects on sperm quality indices of Barbus sharpeyi by image J software

Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS ۱ and ۲) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS ۱ (۴۵mM NaCl, ۵mM KCl, ۳۰mM Tris, ۱۸۸±۲.۳ mosmol/kg) and SAS ۲ (۵۰mM NaCl, ۳۰mM KCl, ۳۰mM Tris, ۱۸۹±۱.۷ mosmol.kg) were added to a mixture of ۱۲ male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS۲ had the highest VCL (۲۱.۳۷±۰.۳۶ µm/s-۱), VSL (۱۱.۶±۰.۳ µm/s-۱) and motility percentage (۸۵.۷۵±۱.۵۶ %) among treatments. The highest A type spermatozoa percentage was recorded for SAS۱ (۴۷.۱۶±۳.۱۵ %) and SAS۲ (۴۳.۸۹±۴.۵۴%). The lowest C type spermatozoa percentage was recorded using SAS۲ (۱۴.۲۴±۱.۵۶ %). Also, the highest fertilization rate was achieved from SAS۲ (۷۹.۵±۰.۳۷%) and SAS۱ (۷۷.۷۴±۴%) respectively. Furthermore the lowest larval deformity rate (۱۳.۷۷±۱.۰۳%) was shown due to the use of SAS۲. Final conclusion confirmed that SAS۱ and SAS۲ have positive and significant effects (P < ۰.۰۵) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran

Keywords:

Sperm , Saline activator solution , Fertilization , Barbus sharpeyi , Iran