Determination of lethal concentration (LC۵۰) values

In this study, lethal concentration (LC۵۰) values of cinnamon hydrosol (Cinnamomum zeylanicum) on carp (Cyprinus carpio) were investigated. In practice, experimental setup was constituted ۳۰ fish (a total of ۱۸۰ fish with ۳۰ control fish) to be placed in three replicates. Hydrosol was added into aquariums at the doses of ۰.۵, ۱, ۲.۵, ۵ and ۱۰% and determined mortality times of carp exposed to these concentrations. Percentage death of fish calculated in these concentrations. Mortality was observed at all treatments exception of dose of ۰.۵ %. The results indicate that the hydrosol had swimming changes, lethargy, lack of breath and leaning to the depth of the aquariums at all of the concentrations. The results of regression analysis indicated that the mortality rate (Y) is positively correlated the concentration (X) having a regression coefficient (R) close to one in each case. While the ۱ h LC۵۰ value (with ۹۵% confidence limits) of the safe dose of C. zeylanicum hydrosol was estimated at LC۵۰=۴.۳۹%, ۲ h LC۵۰ value=۲.۶۲۹%, and ۱۲ h LC۵۰ value= ۱.۰۲۷%.

Keywords:

Hydrosol , Cinnamomum zeylanicum , Mortality , Cyprinus carp