Toxicity comparison of colloidal silver nanoparticles in various life stages of rainbow trout (Oncorhynchus mykiss)

Recognizing the significance of the life stage of fish for nano-eco-toxicological studies, the acute toxicity of colloidal silver nanoparticles (AgNPs) was tested in three different life stages of rainbow trout. Fishes were exposed to colloidal AgNPs at nominal concentrations of ۱۰۰, ۳۲, ۱۰, ۳.۲, ۱, ۰.۳۲, ۰.۱, and ۰.۰۳۲ mg/L. The estimated ۹۶ hr LC۵۰ values were ۰.۲۵, ۰.۷۱, and ۲.۱۶ mg/L for the eleutheroembryos, larvae and juveniles, respectively, revealing a higher sensitivity for the early life stages. In addition, a dose-dependent blood plasma reduction of chloride and potassium, also increase of cortisol and cholinesterase were observed in the juveniles to exposed AgNPs when compared to the controls. Thus, colloidal AgNPs should be classified as “very toxic” and “toxic” to the eleutheroembryo-larva and juvenile stages, respectively, meaning that the release of nanosilver into the aquatic envi ronment or its direct application as an antimicrobial agent in aquaculture should no longer be allowed.

Keywords:

Silver , Nanoparticles , Nanotoxicology , Rainbow trout , Life stage , Blood plasma