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Paper
Title

The Effect of Betaine on Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells In Vitro

بیست و یکمین کنگره پزشکی تولید مثل و شانزدهمین کنگره زیست شناسی و فناوری سلول های بنیادی
Year: 1399
COI: RROYAN21_051
Language: EnglishView: 72
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

TS Tabatabai - Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Scienes, Damghan University, Damghan, Iran
M Haji Ghasem Kashani - Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Scienes, Damghan University, Damghan, Iran
R Maskani - Department of Medical Sciences, Faculty of Paramedical Sciences,Shahroud University, Shahroud, Iran
M Nassiri - Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Scienes, Damghan University, Damghan, Iran

Abstract:

Objective: Human adipose- derived stem cells (hASCs) are af-fected by many biochemical and biophysical stimuli in vivo. Betaine (a dietary supplement) is a biochemical stimulant that promotes the osteogenic differentiation by increasing the entry of calcium into the cell. In this study, the effect of Betaine on the expression of osteogenic genes was evaluated.Material and Methods: hASCs were extracted from abdomi-nal adipose tissue, and stem cells were confirmed by flow cytometry. The experimental groups were: cultured cells in osteogenesis differentiation medium (positive control), in os-teogenesis differentiation medium and Betaine ۱۰ mM (BET + OD), in Betaine (BET) and negative control. On day ۲۱, alka-line phosphatase (ALP) activity and expression of osteogenic genes (RUNX۲ and OCN) were assessed by Real time PCR.Results: A significant increase in ALP activity in BET group was detected compared to OD + BET and positive control. On day ۷, there was a significant increase in ALP activity com-pared to ۱۴-day treatment. There was a significant decrease in the mRNA level of RUNX۲ in BET group compared to positive control. The expression of OCN gene in BET group was signifi-cantly increased compared to the OD + BET, while a significant increase of this gene was seen in positive control compared to OD + BET. There was no significant difference between BET and positive control.Conclusion: The induction of hADSCs with Betaine increased ALP activity and OCN gene expression and decreased RUNX۲ gene expression. The effect of Betaine on OCN gene expression was similar to osteogenesis differentiation medium. Betaine had effective role on ALP activity at an earlier time post induction.

Keywords:

Human Adipose-Derived Stem Cells , Betaine , Osteo-genic Genes , Real Time PCR ,

Paper COI Code

This Paper COI Code is RROYAN21_051. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1421332/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Tabatabai, TS and Haji Ghasem Kashani, M and Maskani, R and Nassiri, M,1399,The Effect of Betaine on Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells In Vitro,21st congress on reproductive biomedicine and 16th congress on stem cell biology & technology ,Tehran,https://civilica.com/doc/1421332

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Type of center: دانشگاه دولتی
Paper count: 1,688
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