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Paper
Title

Using Microwell Chip for Scalable Generation of Pancreatic Aggregates

بیست و یکمین کنگره پزشکی تولید مثل و شانزدهمین کنگره زیست شناسی و فناوری سلول های بنیادی
Year: 1399
COI: RROYAN21_054
Language: EnglishView: 75
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

M ZABIHI - Department of Genetics, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran . Department of Stem Cells and Developmental Biology, Cell Science Research Center , Royan Institute for Stem Cell Biol
Z Ghezelayagh - Department of Stem Cells and Developmental Biology, Cell Science Research Center , Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Department of Developmental Biology, Faculty of Basic Sciences and Advanced Technologies in Biolo
I Zarkesh - Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
M Vosough - Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Y Tahamtani - Department of Stem Cells and Developmental Biology, Cell Science Research Center , Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran,Department of Diabetes, Obesity and Metabolism, Cell Science Research Center, Royan Institute for

Abstract:

Objective: For the production and bio-banking of organoids in large quantities for drug screening and cellular therapies, well-defined procedures for scalable production are required. As well, based on previous studies, co-culture with native pan-creatic mesenchyme promotes self-renewal and expansion of their epithelial progenitors. Therefore, in this study we used a microwell platform as a high throughput three-dimensional (۳D) culture system where human embryonic stem cells derived pancreatic progenitors (hESC-PPCs) can be co-cultured with human fetal pancreatic mesenchyme (hFP-MCs) for large-scale generation of pancreatic aggregates. The advantage of this plat-form compared to conventional suspension culture is its repro-ducibility.Materials and Methods: Non-adherent agarose microwell chip was fabricated by mold-replication technology which con-tained a structured polydimethylsiloxane (PDMS) surface with a standard array of ۴۰۰ µm diameter pyramidal microwells. The PPCs differentiation was achieved from Royan hESC lines. HFP-MCs were isolated from ۱۲-۲۰ week human embryos. For aggregate formation, cell mixture suspension of hESC-PPCs and hFP-MCs was seeded on microwell chips. After aggregates generated by force aggregation and ۲۴ hour incubation, were aspirated from the microwells and placed in static suspension culture.Results: This platform produces approximately ۴۷۰۰ aggre-gates where each aggregate has a diameter of ۷۰–۱۲۰ µm, lead-ing to uniformly sized, shaped and stable aggregates with low variation in diameter. We demonstrated that both cells are pre-sented inside the aggregates after ۴۸ hours of suspension cul-ture and formed aggregates can be harvested easily without any additional forces, which prevent damage to aggregates during harvesting. Aggregates were expressing PP- and MC specific gene and protein markers after ۴۸ hours culturing.Conclusion: Altogether, pancreatic aggregates which are pro-duced by our method formed round-shaped with well-defined borders aggregates and were able to be maintained stable in culture for short-range period in order to apply in in vivo trans-plantations and cell therapies.

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This Paper COI Code is RROYAN21_054. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1421335/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
ZABIHI, M and Ghezelayagh, Z and Zarkesh, I and Vosough, M and Tahamtani, Y,1399,Using Microwell Chip for Scalable Generation of Pancreatic Aggregates,21st congress on reproductive biomedicine and 16th congress on stem cell biology & technology ,Tehran,https://civilica.com/doc/1421335

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Type of center: موسسه غیرانتفاعی
Paper count: 1,587
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