Mohammad Ghodsi - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Vida Hojati - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Armin Attaranzade - Imam Reza Hospital, Mashhad University of Medical Science, Mashhad, Iran.
Bita Saifi - Department of Anatomy, Faculty of Medicen, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran.

Background: Endometriosis is associated with abnormal immunologic responses and combined inflammatory and anti-inflammatory conditions. Objectives: This study aims to investigate follicular fluid (FF) concentration of interleukin (IL)-۳, IL-۵, and IL-۶ in women with and without endometriosis. Materials and Methods: In this a cross-sectional study ۶۸ women who were referred to the in vitro fertilization center of Imam Reza hospital in Mashhad during ۲۰۱۸ was selected randomly. Leaves of cytokines in the FF samples were evaluated in the endometriosis and the control group (n = ۳۴/each). The diagnostic accuracy of cytokines and clinical characteristics were evaluated. Results: IL-۳ and IL-۶ were significantly changed in FF of the women with endometriosis compared with control group (p = ۰.۰۴, and p = ۰.۰۰۳, respectively), the mean concentration of IL-۵ in the endometriosis group was lower than the control group (p = ۰.۵). But it was not significant. There were significant differences in the menstrual cycle, dyspareunia, and dysmenorrhea between the groups (p = ۰.۰۰۳, p = ۰.۰۴, and p = ۰.۰۲, respectively). The diagnostic accuracy of IL-۳ and IL-۶ in the FF was low, with the area under the curve of ۰.۶۱۴ and ۰.۶۴۵, respectively. Conclusion: Although none of the cytokines had a predictive value for endometriosis, the decreased levels of IL-۳ and increased levels of IL-۶ in the FF samples of women with endometriosis, and risk factors, including irregular menstrual cycle, dyspareunia, and dysmenorrhea, could be associated with the pathogenesis of this painful disease.

Interleukin-۳, Interleukin-۵, Interleukin-۶, Follicular fluid, Endometriosis., اینترلوکین-۳, اینترلوکین-۵, اینترلوکین-۶, مایع فولیکولی, آندومتریوز.