Lysophosphatidic acid supports the development of vitrified ovarian follicles by decreasing the incidence of cell death: An experimental study
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 156
This Paper With 16 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJRM-20-4_003
تاریخ نمایه سازی: 18 اردیبهشت 1401
Abstract:
Background: Lysophosphatidic acid (LPA) contributes to follicular activation, oocyte maturation, in vitro fertilization, and embryo implantation.
Objective: This study was designed to evaluate the effects of LPA to improve the development of isolated follicles derived from whole mouse cultured vitrified ovaries.
Materials and Methods: In this experimental study, first, the ۱-wk-old mouse ovaries in the non-vitrified and vitrified groups were cultured in the presence of ۲۰ µM of LPA for ۱ wk. Then, their isolated preantral follicles were cultured individually for ۱۲ days in the presence or absence of ۴۰ µM of LPA. The following evaluations were done for the cultured follicles: a viability test using Calcein AM staining, flow cytometry using annexin V/Pi, and analysis of the expression of genes by real-time reverse transcription polymerase chain reaction. The maturation rates of the oocytes were compared among groups and some of the released metaphase II oocytes were subjected to in vitro fertilization.
Results: In all LPA treated groups, the rates of survival and follicular development were higher, and the incidence of cell death and expression of pro-apoptotic genes were lower, than in the non-LPA supplemented groups (p = ۰.۰۳۵). There was no significant difference between the vitrified and non-vitrified groups regarding follicular or oocyte development, but the expression of Bad and LPA receptors genes was significantly altered in the vitrified LPA supplemented group in comparison with the non-vitrified LPA supplemented group (p = ۰.۰۲۸).
Conclusion: LPA improved the survival and developmental potential of the isolated follicles. Despite some alterations in the expression of apoptosis-related genes in the vitrified ovaries, LPA had positive effects on the survival and development of these follicles.
Keywords:
Cell death , In vitro oocyte maturation , Lysophosphatidic acid , Lysophosphatidic acid receptors , Ovarian follicles , Vitrification. , مرگ سلولی , بلوغ تخمک در شرایط آزمایشگاهی , اسید لیزوفسفاتیدیک , گیرنده های لیزوفسفاتیدیک اسید , فولیکول های تخمدان , انجماد.
Authors
Neda Abedpour
Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Anatomy Department, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Nassim Ghorbanmehr
Biotechnology Department, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Mojdeh Salehnia
Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :