Wound Healing Effect of Hydroalcoholic Extract of Salvadora Persica (Miswak) on Physicaly Induced Second-Degree Burn Wound in BALB/c Mice

Introduction: Different Scholars have mentioned a lot of benefits for Salvadora Persica(SP) compounds. The Present study was conducted aiming at evaluating the effect of hydroalcoholic extract of SP on second-degree burn healing in Mice.Materials and Methods: The present study population was ۶۰ mature mice (BALB/c). The mice were divided into ۵ equal groups. Groups ۱ and ۲ were treated by means of the application of ۵% hydroalcoholic extract of SP and ۱۰% extract of it, respectively; regarding group ۳ revieved silver sulfadiazine ointment. Group ۴ was treated using Vaseline. Group ۵ received no treatment. Then, samples from the injured sites was gathered, and wound healing was evaluated histopathologically.Results: Inflammation and infilteration of nutrophils and lymphocytes in the treatment groups, i.e. the groups to which ۵% and ۱۰% SP extract was applied, demonstrated a significant decrease, compared to the vaseline and sham groups (P<۰.۰۱). Moreover, the number of fibroblasts increased followed by collagen production, epithelialization and new hair follicles formation at the wound margins on the ۱۰th and ۱۴th day in the ۵% and ۱۰% SP extract groups significantly increased compared to vaseline and sham groups (P<۰.۰۵). The number of fibroblasts and the density of collagen tissues in the group treated with ۱۰% SP extract even showed a significant increase compared to the groups treated with ۵% SP extract and silver sulfadiazine on the ۱۴th day.Conclusion: It was found that hydroalcoholic salvadora Persica extract accelerated the healing of second-degree burn injuries in BALB/c mice.

Keywords:

Salvadora Persica , Wound , Burn , Skin , Mice