Identification of Mycoplasma Muris Isolated from Vaginal Samples of NIH Mice

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_MML-2-1_003

تاریخ نمایه سازی: 25 اردیبهشت 1401

Abstract:

Mycoplasma muris (M.M) is a small pathogenic bacterium that lives in the female mouse genital tract. Mycoplasma muris may have harmful effects on the reproductive health of female. This research was performed to optimize the detection of M. muris in NIH mice in the Department of Animal Breeding, Razi Vaccine and Research Institute, Iran. In this cross-sectional study, ۲۹ vaginal samples of NIH mice were selected through simple random sampling. For detection of the mycoplasma, the vaginal tissue removal of samples was done. First, samples were crushed using mortar and pestle with PBS ۱ml, then were cultured in the PPLO broth and incubated at ۳۷°C for ۲۴h, they were passed through ۰.۴۵ μm pore-size filters and inoculated into specific PPLO broth and agar media for ۳-۴ weeks. In the next section, the PCR test was used with primers of ۱۶S rRNA gene of M. muris. From ۲۹ tested samples, ۱۷.۲۴% samples were positive for M. muris by PCR method, while ۳۵.۹۳% cultures showed positive. The phylogenetic analysis indicated a new strain of M. muris. The results of culture and PCR methods displayed the contamination in NIH mice. Therefore, Therefore, more researches are needed regarding the presence of mycoplasma for treatment and clinical signs.

Authors

Mohammad Reza Zinatizadeh

Department of Genetics, Faculty of Science, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran

Fatemeh Abedini

Department of Animal Breeding, Razi Vaccine and Serum Research Institute, Karaj, Iran

Mostafa Jafarpour

Department of Microbiology, Faculty of Science, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran

Zahra Masoumalinejad

Department of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran

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