Gabriel Cardia - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil
Francielli Silva-Comar - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil
Carla Bonetti - Department of Biochemistry, State University of Maringá, Maringá, PR, Brazil
Edvalkia Rocha - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil
Mayara Zagoto - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil
Valeria Amaral - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil
Livia Bracht - Department of Biochemistry, State University of Maringá, Maringá, PR, Brazil
Saulo Silva-Filho - Pharmaceutical Sciences, Food and Nutrition College, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil
Ciomar Bersani-Amado - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil
Roberto Cuman - Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil

Objective: In the present study, the hepatoprotective effects of β-myrcene (MYR) on acetaminophen-induced hepatotoxicity were investigated.Materials and Methods: A total of ۴۰ Balb/c mice were randomly divided into five groups as follows: ۱) Normal control group which received only carboxymethylcellulose (CMC), the vehicle used to dissolve acetaminophen (N-acetyl-p-aminophenol, APAP, paracetamol) and MYR; ۲) APAP group which received a single dose of acetaminophen (۲۵۰ mg/kg) orally on day ۷; ۳) Silymarin group which received ۲۰۰ mg/kg/day of silymarin; and ۴ and ۵) pretreatment groups in which, mice were treated with ۱۰۰ or ۲۰۰ mg/kg/day of MYR. Liver and blood samples were collected to analyze serum aminotransferases, inflammatory response, oxidative stress markers, and histopathological insults.Results: Our results showed that MYR pretreatment attenuated liver damage and restored liver cells function and integrity as it decreased the leakage of serum aminotransferases (alanine and aspartate aminotransferases (ALT and AST, respectively)) into the blood (p<۰.۰۱). MYR treatment also reduced levels of myeloperoxidase (MPO) activity and nitric oxide (NO) (p<۰.۰۰۱). In addition, MYR pretreatment demonstrated significant antioxidant activity by decreasing malondialdehyde (MDA), reactive oxygen species (ROS), and reduced glutathione (GSH) levels (p<۰.۰۰۱). Furthermore, it restored the hepatic level of superoxide dismutase (SOD), catalase (CAT), and oxidized glutathione (GSSG) (p<۰.۰۰۱). Conclusion: For the first time, our results showed that MYR treatment significantly improved liver function by reducing oxidative stress and the inflammatory response induced by APAP.

β-myrcene, Antioxidants, Acute hepatic failure, Liver diseases