Rodrigo Vargas-Ruiz - Center of Biomedical Research of the South, Mexican Institute of Social Security, Morelos, México. Section of Postgraduate Studies and Research, Superior School of Medicine, National Polytechnic Institute, Mexico City, México
Jose Eduardo Roa-Coria - Section of Postgraduate Studies and Research, Superior School of Medicine, National Polytechnic Institute, Mexico City, México
Arturo Mora-Olivo - Institute of Applied Ecology, Autonomous University of Tamaulipas, Tamaulipas, México
Alejandro Zamilpa - Center of Biomedical Research of the South, Mexican Institute of Social Security, Morelos, México
Maribel Lucila Herrera-Ruiz - Center of Biomedical Research of the South, Mexican Institute of Social Security, Morelos, México
Rosa Issel Acosta-Gonzalez - Reynosa-Aztlan Multidisciplinary Academic Unit, Autonomous University of Tamaulipas, Tamaulipas, México
Rosa Mariana Montiel-Ruiz - Center of Biomedical Research of the South, Mexican Institute of Social Security, Morelos, México

Objective: This study was conducted to investigate the antinociceptive and anti-inflammatory effect of ethyl acetate fraction of Oenothera rosea (EAOr) and the mechanism involved, in mice.Materials and Methods: The antinociceptive activity was tested using chemical- and heat-induced nociception models. The anti-inflammatory activity was tested using carrageenan-induced edema and inflammatory cytokines were measured.Results: EAOr reduced the licking time on the second phase of the formalin test (۱۰۰ and ۱۷۷ mg/kg). The antinociception of EAOr was prevented by L-NAME (۱۰ mg/kg), ۱H-[۱, ۲, ۴]-oxadiazolo [۴, ۳-a]-quinoxalin-۱-one (ODQ, ۰.۱ mg/kg), glibenclamide (۱۰ mg/kg) and bicuculline (۱ mg/kg), but not by naloxone (۲ mg/kg). Also, EAOr decreased licking time in capsaicin induced-nociception. EAOr did not have effect on withdrawal latency in tail-flick test. Carrageenan-induced paw edema was reduced by EAOr, and TNF-α and IL-۱β levels were reduced in mice treated with EAOr by ۷۲.۲ and ۳۲.۸%, respectively. Furthermore, EAOr did not present side effects as sedation nor gastric injury. Chemical analysis of this fraction showed the presence of glycosylated quercetin derivatives such as quercetin glucoside and quercetin rhamnoside in a ۲.۵% concentration.Conclusion: This study demonstrates antinociceptive and anti-inflammatory effect of an organic fraction of O. rosea and its possible interaction with the NO-cGMP-K+ channels and GABAergic system and thus, it could be considered a therapeutic alternative.

Oenothera rosea, Nociception, Inflammation, GABA, Nitric oxide, cGMP