PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection

Exotoxin A (exoA) is a major pathogenic factor in Pseudomonas aeruginosa. This non-fermenting gram-negative bacillus causes a wide range of infections. P. aeruginosa is a major cause of hospital-acquired infections. With this view, this study was aimed to identify P. aeruginosa strains carrying the exoA gene isolated from burn wounds. As part of the cross-sectional study, ۱۱۰ samples of burns were collected in several hospitals. After identification of bacterial strains by biochemical and microbiological tests, genomic DNA was extracted. PCR reaction was performed for screening for exoA gene. After examining DNA extracted using quantitative (Nano drop with OD۲۶۰/۲۸۰=۱.۸-۲nm) and qualitative (electrophoresis on the ۰.۷% gel agarose) methods, ۷۵.۴% (n; ۸۳ of ۱۱۰) isolates carried the exoA gene. The high prevalence of the exoA gene in P. aeruginosa strains indicate the important role of this exoenzyme in the spread of infection in burn patients. Therefore, it is recommended that people with burn infections be screened for the exoA gene.   Please cite this article as: Gholamreza Morshedi, Vahid Ghanbarinejad, Mina Zarei, Abazar Pournajaf, Hossein Hassanian-Moghaddam, Vahid Hadi,Abbas Isvand, Mohammad Reza Saadati, Homayoun Maleki, Majid Keshavarzi. PCR based identification of exotoxin A-producing Pseudomonas aeroginosa isolated from burn wound infection. Trends in Pharmaceutical Sciences. ۲۰۲۲;۸(۱):۵۱-۵۶ .doi: ۱۰.۳۰۴۷۶/TIPS.۲۰۲۱.۹۳۲۱۸.۱۱۲۱

Keywords:

exotoxin A , Pseudomonas Aeruginosa , Burn Wound