Ruaa Ali Muhammed Ali - The Gifted Students’ School in Najaf, Ministry of Education, Iraq.
Jamal Mohammed Ridha Alshara - Faculty of Dentistry, University of Kufa, Kufa, Iraq.
Nabil Salim Saaid Tuwaij - Faculty of Science, University of Kufa, Kufa, Iraq.
Huda Jameel Baker Al-khilkhali - Faculty of Science, University of Kufa, Kufa, Iraq.

Background: Escherichia coli (E. coli) remains one of the leading agents of urinary tract infection (UTIs), it has become resistant to many drugs. Current work aimed to evaluate some chemical substances as antibacterial agents and molecular study of virulence factors associated with UTIs. Methods: This work involved ۱۳۳ urine specimens obtained from females’ patients suffering from UTIs, Methods of well diffusion and disk diffusion were achieved to assay the effect of some chemical substances and antibiogram profiles toward Sulfamethoxazole-trimethoprim (SXT)-resistant E. coli respectively. Virulence genes were done based on the technique of Polymerase Chain Reaction (PCR). Results: The results recorded ۴۹/۱۳۳ (۳۶.۸۴%) E. coli among women suffering UTIs, ۲۸/۴۹ (۵۷.۱۴%) were resistant to SXT drug. imipenem, meropenem, and nitrofurantoin were recorded more effectively. Chemicals substances at the concentration ۰.۳ (g/ml) recorded percentages of inhibition, reaching ۹.۱۴۳±۱.۴۴۲, ۱۵.۳۶±۰.۵۹۱۴, and ۲۱.۸۲±۰.۸۶۹۹ for NaHCO۳, Ch۴c, and Viroxide Super™ respectively. PCR demonstrated that ۲۸/۲۸ (۱۰۰%) of SXT-resistant E. coli isolates were harbored Sul-۲, FeoB and PapC genes, while ۱۴/۲۸ (۵۰%), ۱۵/۲۸ (۵۳.۵۷%), ۱۹/۲۸ (۶۷.۸۵%) and ۲۶/۲۸ (۹۲.۸۵%) in U۲۵۰ (pet), FumC, Sul-۱ and IutA genes, respectively. Sul-۳ gene was not observed. Conclusions: Observed a high percentage of E. coli that were resistant to SXT drug, and having several virulence genes, poses a real threat, it requires a real pause to create substitutions to limit the spreading of this threat.

Chemical substance, PCR, SXT-resistant E. coli, UTIs.