Synthesis of MnFe۲O۴ nanoparticles for adsorption of digestive enzymes: Kinetics, isothermal and thermodynamics studies

The role of enzyme engineering in biotechnology, biological and pharmaceutical process cannot be over emphasized. This study compared the adsorption of amylase and protease on to manganese ferrite (MnFe۲O۴). The metal ferrite was synthesized via a sol – gel technique and characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), Electron paramagnetic resonance (EPR) and Fourier transform infrared spectroscopy (FTIR). The adsorption was study in a batch process and the data were subjected to kinetics and isotherm models. The pseudo-first order best fitted the kinetic data with R۲> ۰.۹۹. The data were fitted well by the entire isotherm models considered with the maximum adsorption isotherm of ۱.۶۰۲ and ۷.۳۳۰ mg/g.  The thermodynamic parameters give negative Gibb’s free energy, ΔG, showing a spontaneous adsorption; positive ΔH indicated an endothermic favoured process, while ΔS values showed that the process progress with lower entropy change.

Keywords:

Adsorption , Amylase , Isotherm , Manganese Ferrite , Protease