Synergetic signal amplification of multi-walled carbon nanotubes-Cetyltrimethylammonium Bromide and Poly-L-Arginine as a highly sensitive detection platform for Rutin

In this research, a glassy carbon electrode was coated with a thin layer of multi-walled carbon nanotubes in the presence of the surfactant and subsequently was electro-polymerized with Poly-L-arginine (P-L-Arg). The prepared electrode was used as an effective sensor for the quantitative detection of Rutin (Ru). The fabricated electrode exhibited good electrochemical performance with low electron transfer resistance. The electrochemical behavior of Ru at the prepared electrode was also investigated by cyclic voltammetry and differential pulse voltammetry techniques. For modified glassy carbon electrode, the transfer coefficient (α), the number of electrons involved in the rate-determining step (nα) and electron transfer rate constant (ks) calculated. Under the favorable conditions, a linear relationship between the oxidation peak current and concentration of Ru was obtained in the range from ۰.۱ µM to ۱۰ µM with a detection limit of ۰.۰۴۸ µM and with the extraordinary high sensitivity value of ۶.۳۷۶۷, µA/µM was obtained. Interference and stability studies showed that satisfactory detection results are achieved using this electrode. The proposed electrode was successfully applied for the determination of Ru in human blood serum samples.

Keywords:

Modified electrode , Multi-Walled Carbon Nanotube , Poly-L-Arginine , Rutin , Voltammetry