Atousa Zia - Faculty of Pharmacy, Islamic Azad University, Tehran, Iran
Hakimeh Rezaei Aghdam - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Mostafa Saffari - Faculty of Pharmacy, Islamic Azad University, Tehran, Iran
Jafar Farzaneh - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Morteza Pirali Hamedani - Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Seyed Esmaeil Sadat Ebrahimi - Pasteur Institute of Iran
Kimia Abdollahi - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Saghar Sedigh Kheirabad - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Fatemeh Zarringhalam - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Farnoor Davachi omoomi - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Ramin Farhoudi - Pasteur Institute of Iran
Mehdi Shafiee Ardestani - Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Objective(s): Today, cancer is one of the health concerns in modern societies. The use of nanoparticles in medical science has created new possibilities for diagnosis, imaging of tumors, and treatment of cancer in humans. Materials and Methods: In this study, chitosan nanoparticles modified with quinic acid were used to diagnose breast cancer using a single-photon emission computed tomography imaging technique. For this purpose, quinic acid was activated by EDC/NHS and then binding was performed by adding chitosan nanoparticles. Results: TEM, DLS, FTIR, and LC/MASS analyzes were used to investigate this conjugation. MTT toxicity test showed no toxicity on the HEK-۲۹۳ cell lines and therapeutic properties for the MCF-۷ cell line. The response surface method depends on the central composite design that was applied. For the highest labeling efficiency, using ۳ factors: the amount of chitosan-quinic acid, pH, and SnCl۲ as a reducing agent, and the efficiency of labeling was evaluated by thin-layer chromatography (TLC). Conclusion: Finally, the study of biodistribution using SPECT imaging in cancer mice and its comparison with blocking tests showed that this compound has a good ability to diagnose breast cancer.

Blocking, Breast Cancer, Chitosan, Quinic acid, SPECT imaging