Direct Detection of Phanerochate chrysosporium, Engyodontium album, and Fusarium venenatum in Soil Samples Collected from Different Regions of Iran by PCR Method

Introduction: Engyodontium album, Fusarium venenatum, and Phanerochaete chrysosporium fungi have an important role in the production of proteinase K, Quorn mycoprotein, and bioremediation, respectively. There are several techniques for the detection of fungi in soil. The purpose of this study was to detect and identify E. album, F. venenatum, and P. chrysosporium, directly from soil using the Polymerase Chain Reaction (PCR) method.Materials and Methods: A total of ۲۴۰ soil samples were collected from different regions of Iran, including Tehran, Zanjan, Hamadan, Kermanshah, Kurdistan, and Hormozgan Provinces. The DNA was extracted and purified directly from soil samples with the modified phenol-chloroform method and by PVPP (Polyvinylpolypyrrolidone) column, respectively. The PCR method was performed using designed specific primers. The PCR products of the E. album, F. venenatum and P. chrysosporium with an approximate size of ۲۴۸, ۲۰۲, and ۵۰۲ bp were sequenced, respectively.Results: In this study two isolates of P. chrysosporium, ۱ isolate of F. venenatum, and ۱ isolate of E. album were identified. The fungi detected with specific primers in soil samples were compatible with the results of sequencing.Conclusions: This investigation described a reliable method that can be used to detect important fungi in the industries and biotechnology directly in soil using specific primers. The results can provide an appropriate platform for next applied research and mass production of valuable fungal products in industries and biotechnology. 

Keywords:

Engyodontium album , Fusarium venenatum , Phanerochaete chrysosporium , Soil , PCR