Analysis of Expression Profiles of GLI۳A, LATS۲ and MOB۱A genes in Patients with Congenital hypothyroidism

In recent years, congenital hypothyroidism (CH) has been reported as the most prevalent endocrine disorder and most common cause of preventable mental retardation  in many parts of the world, especially in Asia. Delays in early diagnosis and treatment of CH can lead to growth retardation, as well as neurological and psychological disorders. Many genetic defects along with their molecular mechanisms based on pathophysiology have not been identified in many babies with congenital hypothyroidism. This study aimed to evaluate the expression of GLI۳A and LATS۲, MOB۱A genes in patients with congenital hypothyroidism. In this study, the expression pattern of GLI۳A, LATS۲, and MOB۱A genes after RNA extraction and converted to cDNA from the blood of ۲۰ patients and ۲۰ control samples were examined by the Real-time quantitative PCR (qRT-PCR). The obtained data were analyzed using Spss software and Mann-Whitney statistical method. Statistical analysis of findings has shown significant differences in the expression of GLI۳A gene in two groups of patients and control. However, in the study on the expression of two genes LATS۲ and MOB۱A, there was no significant difference, so it can be assumed that expression of GLI۳A gene may play a role in risk of congenital hypothyroidism while changes in expression of LATS۲ and MOB۱A genes are not involved in this disease. Results of the present study demonstrated that GLI۳A gene expression could be a genetic risk factor for congenital hypothyroidism. In the present study, LATS۲ and MOB۱A gene expression did not show significant risk for susceptibility CH disease.

Keywords:

Expression , GLI۳A , MOB۱A , LATS۲ , Congenital hypothyroidism , Real-time PCR