Phytochemical Analysis and Antimicrobial Evaluation of Quince Seeds’ Extracts

Microbial infection and microbial resistance to the classical antibiotics have been immense challenges which portend the health of societies. This has directed researchers to straight their attention onto the plants to discover new possible antimicrobial compounds. In this work, ۵ solvents were employed to extract the dried seeds powder of quince by ultrasound including ethanol, ethyl-acetate, chloroform, n-hexane, and diethyl-ether. Each extract was subjected to three in vitro antimicrobial tests using the broth-dilution method. The antibacterial effect against the following aerobic bacteria which are Pseudomonas aeruginosa ATCC ۲۷۸۵۳, Klebsiella pneumonia ATCC ۷۰۰۶۰۳, Haemophilus influenzae ATCC ۴۹۲۴۷, Escherichia coli ATCC ۲۵۹۲۲, Salmonella typhi ATCC ۶۵۳۹, Shigella dysenteriae ATCC ۱۳۳۱۳, and non-pathogenic Escherichia coli BAA-۱۴۲۷ applying ciprofloxacin antibiotic as a standard reference, antibacterial study against anaerobic bacteria which include Bacteroides fragilis ATCC ۲۵۲۸۵, Clostridium perfringens ATCC ۱۳۱۲۴, Fusobacterium necrophorum ATCC ۲۵۲۸۶, and Prevotella melaninogenica ATCC ۲۵۸۴۵ employing metronidazole as a reference, and antifungal study versus Candida albicans ATCC ۱۰۲۳۱ and Aspergillus niger ATCC ۱۶۸۸۸, nystatin was the reference. The toxicity against the normal bacterial flora was evaluated to verify the safety profile of the extracts. The results revealed that the extracts have antimicrobial activities with supremacy linked to ethanol extract. In accordance to the activity evidence values, the extracts displayed bactericidal and fungicidal activities, with ethanol extract having the least harmful effect against non-pathogenic microbial strain.

Keywords:

Quince , Seeds extract , Antimicrobial , Broth dilution