A. Zambare - Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-۶, CIDCO, Aurangabad (MS) India.
T. Nerpagar - Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-۶, CIDCO, Aurangabad (MS) India.
N. Chaudhari - Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-۶, CIDCO, Aurangabad (MS) India.
P. Manchalwad - Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-۶, CIDCO, Aurangabad (MS) India.
S. Harke - Department of Biotechnology, MGM’s Jawaharlal Nehru Engineering College, MGM Campus, N-۶, CIDCO, Aurangabad (MS) India.

In study, spherical Silver nanoparticles (SNPs) were synthesized by chemical reduction method from a metal precursor silver nitrate in presence of an anionic surfactant and strong reducing agent. In this experimental work, SNPs are synthesized in presence of different concentration of stabilizing agent and effect of stabilizing agent on size distribution of SNPs have been observed. Further antibacterial activities of the SNPs prepared under different concentration of sodium lauryl sulphate (SDS) carried out on gram negative and gram positive bacteria showed that as concentration of sodium lauryl sulphate increases antibacterial activity decreases.

chemical reduction, Silver, Colloidal spherical silver nanoparticles, Sodium lauryl sulphate, Antibacterial activity, Gram positive and Gram negative bacteria