P. Yarmohamadi - MSc of Animal Physiology, Faculty of Sciences , University of Shahrekord, Shahrekord, Iran.
M. Arabi - Department of Biology, Faculty of Sciences, University of Shahrekord, Shahrekord, Iran.

Silver salts and nanosilver (NS) are the common antimicrobial used in the treatment of chronic wounds, so they are important to assess their dermal toxicity. Therefore, in this study, the possible dermal toxicity of NS particles and silver nitrate (AgNO۳), was tested by determining the serum level of transforming growth factor-β۱(TGF-β۱) and study on of mouse skin tissue. In this study, ۳۰ BALB/c mice were randomly allocated into NS, AgNO۳ and control groups (n=۱۰). After general anaesthesia and shaving the back of all animals in near the vertebral column, in NS group, a volume of ۵۰µl ۱۰ µg/ml of nanosilver solution (۴۰ nm), in AgNO۳ group, with the same amount of AgNO۳ solution (۱۰۰ µg/ml), in control group, the same amount of distilled water was added to the sterile bandage of mice. The shaved areas were covered with sterile bandage that and fixed with cloth glue. After ۳ and ۷ days, the bandages were opened and serum level of TGF-β۱ was measured using standard kits and preparation of skin samples for histological analysis. Results showed toxicity of NS and AgNO۳ by a significant reduction of TGF-β۱ without any damage to skin.

Nanosilver, Silver nitrate, Dermal toxicity, Skin, TGF-β۱