Comparative analysis of antibacterial activity of Silver nanoparticles synthesized using leaf extract of wheat varieties

In the present study, we have reported the biological synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract prepared by hot percolation treatment of wheat varities (PBW۳۴۳, Triticum durum and Aegilops tauschii )for the reduction of silver ions to silver nanoparticles. Bioreduction of Ag+ to Ag۰ was observed when aqueous extract augmented with silver nitrate (AgNO۳) was incubated for ۲ hours at ۱۰۰۰C. The formation of silver nanoparticles was confirmed by surface plasmon resonance as determined by UV-Vis spectra at ۴۳۰ nm. Silver nanoparticles of size ranging from ۵-۱۴ nm of spherical shape were characterized using transmission electron microscopy (TEM). These nanoparticles were found to possess potential antibacterial activity against (Escherichia coli, Staphylococcus aureus, Klebsiella) using disc diffusion method and macro dilution(tube)broth method. This environmental friendly method provides a simple, easy, fast and cost effective method for nanoparticles synthesis and can be used in several areas of medicines and industries.

Keywords:

A. Tauschii , Antibacterial , Macrodilution (Tube) broth method , Silver nanoparticles , T. Durum , TEM (Transmission Electron Microscopy) , Wheat varieties (PBW۳۴۳)