Salehe Ganjali - Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
Hamideh Khajeh - Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran
Zahra Gholami - Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
Zahra Jomeh-ghasemabadi - Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
Bahman Fazeli-Nasab - Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran

The seeds of most medicinal plants have a variety of dormancy. Therefore, it is necessary to know the effective methods of breaking the seed dormancy for their production and cultivation. In this study, to determine the best method of breaking dormancy and increasing the germination percentage of tattoo seeds, various factors have been investigated. Different treatments, scratching with sandpaper, embryo culture, GA treatment (۱۰۰, ۱۵۰ and ۲۰۰ PPM) and sulfuric acid treatment (۵۰, ۷۵ and ۱۰۰%) with filter paper or on MS medium containing BAP (۰.۵, ۱ and ۱.۵ mg/l) was applied with untreated samples (control). Embryo culture was performed only on MS medium containing BAP hormone. The analysis of variance showed that all interactions with the studied treatments were significantly different in terms of germination percentage, fresh and dry weight of roots and stems (P <۰.۰۱). It was observed that the use of benzyl aminopurine (۱.۵ mg/l) with gibberellic acid (۲۰۰ ppm) increased the seed germination percentage compared to the control. The use of benzylaminopurine (۱.۵ mg/l) and sulfuric acid (۵۰%) significantly increased seed germination compared to the control. It was concluded that the effect of GA with BAP on seed germination was greater than sulfuric acid with BAP.

Datura stramonium L, Dormancy, Benzyl aminopurine, Gibberellic acid, Sulfuric acid