Biochemical and physiological evaluation of At.TC rapeseed (Brassica napus L.) under drought stress

Drought stress is one of the major problems in crop production like rapeseed for two reasons. The first reason is that it reduces the plant growth rate through the alternation in physiological, biochemical and molecular processes. The second reason is that it disrupts the balance between the production of reactive oxygen species and the plant's antioxidant defense activities which leads to oxidative stress. One of the plant defense mechanisms is the use of non-enzymatic antioxidants such as vitamin E. This study aims to investigate several biochemical and physiological parameters in some rapeseed genotypes and their transgenic lines. Nima, Hyola۴۸۱۵, RGS۰۰۳, Dalgan and Zafar genotypes besides transgenic rapeseed plants including Hyola۴۸۱۵ (Line۵ and Line۶) and RGS۰۰۳ (Line۳), are studied at four levels of ۳۰%, ۵۰%, ۷۰%, and ۹۰% field capacity (FC) using a factorial experiment in the frame of Completely Randomized Design (CRD). It is observed that by decreasing FC, the relative water content (RWC) reduces. On the contrary, malondialdehyde (MDA), polyphenol oxidase (PPO) and catalase (CAT) increase.

Keywords:

Drought stress , At.TC gene , Rapeseed , Transgenic plants