Correlation Between IL-۱۳rs۲۰۵۴۱(A> G) Gene Polymorphism and Bronchial Asthma Among Iraqi Patients

Background: Bronchial asthma has a complicated genetic history. Changes in gene expression may be caused by gene polymorphism, cytokines play a central role. IL-۱۳ is an interleukin that has been shown to play a role in the disease's immunopathogenesis. The current study investigated the relationship between rs۲۰۵۴۱ of the IL-۱۳ gene and Bronchial asthma in Iraqi patients. Methods: Seventy-five patient and fifty healthy individuals as a control. The DNA was extracted from blood samples. Detection of genotype IL-۱۳SNP (rs۲۰۵۴۱) were achieved by RFLP-PCR.  Results: indicated a highly significant the levels of the IgE, and IL-۱۳ in the patients compared to control at (p value≤ ۰.۰۱), (۴۵۶.۴۵±۲۹۰.۱۰۶ vs. ۳۰.۰۸±۲۴.۴۱۴), (۵۹.۵۹۸۰±۲۰.۹۳۷۵۰ vs.۶.۷۰۳۴±۴.۱۰۵۴۷) pg/ml respectively. Result shows no significant differences in the frequency distributions of IL-۱۳ SNP (rs۲۰۵۴۱) for all genotypes in cases and controls. A protective role of asthma, (OR: ۰.۶۲; CI.۹۵%: ۰.۲۳ - ۱.۶) and (OR ۰.۸۹; CI.۹۵%:۰.۴۲ - ۱.۸۹) were observed for wild type homozygous and heterozygous genotype respectively. Whereas the AA genotype (۴۲.۷%) in cases and (۳۴.۰%) in control, that (OR:۱.۴۴; CI.۹۵%:( ۰.۶۶ - ۳.۰۷) mutant homozygous were risk factors of asthma among individuals. The genotypes of IL-۱۳ rs۲۰۵۴۱ (GG, AG, AA) among patients and controls were significantly correlated with IgE and IL-۱۳ results at (p≤ ۰.۰۵).  Conclusions: AA genotype in case and control mutant homozygous were risk factors of asthma among individuals. It’s possible that this has a predisposing impact on the development of asthma.

Keywords:

Bronchial Asthma , RFLP , IL-۱۳ , SNP