Maryam Sheikhi - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Maryam Mohammadi-Sichani - Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Background: One of the most important therapeutic challenges is fighting against infectious diseases and developing their bacteriahigh antibiotic resistance. Bacterial resistance is one of the reasons for the growing uses of herbs in treating bacterial infections.Objectives: The purpose of this study was investigating the antibacterial and anti-biofilm properties of the bulb of Urginea maritimaagainst Pseudomonas aeruginosa (ATCC: ۲۷۸۵۳, ATCC: ۹۰۲۷) and Staphylococcus aureus (ATCC: ۲۵۹۲۳, ATCC: ۹۱۴۴).Methods: Methanol and acetone extracts of the bulb were prepared by the Soxhlet method. The antibacterial properties of theextracts were evaluated by the agar well diffusion method, and the minimum inhibitory concentration was evaluated by the microdilutionmethod. The anti-biofilm effect of the extracts was also measured by the microplate technique and by crystal violetdying. Determining the compounds of the U. maritima bulb was done by the GC/MS device.Results: Results showed that acetone extract of U. maritima bulb prevents the growth of standard and clinical strains of P. aeruginosaand S. aureus. The highest antibiofilm effect was related to the methanol extract of the bulb against S. aureus, with the reducing ofbiofilm production of about ۹۴%. Most of the extracted compounds of the U. maritima bulb include xylene, pentane, and phenylethane.Conclusions: The data of this study suggest that methanol and acetone extracts of the bulbs of U. maritima have potential activityas an antibacterial and antibiofilm agent, especially against S. aureus.

Anti-Bacterial Agent, Biofilms, Plant Extract, Urginea maritima, Staphylococcus aureus, Pseudomonas aeruginosa