Application of Multiplex PCR for the Identification of Oxacillinase Genes and Determination of Antibiotic Resistance Pattern in Environmental Isolates of Acinetobacter baumannii in ICU
عنوان مقاله: Application of Multiplex PCR for the Identification of Oxacillinase Genes and Determination of Antibiotic
Resistance Pattern in Environmental Isolates of Acinetobacter baumannii in ICU
شناسه ملی مقاله: JR_JCMI-8-3_007
منتشر شده در در سال 1400
شناسه ملی مقاله: JR_JCMI-8-3_007
منتشر شده در در سال 1400
مشخصات نویسندگان مقاله:
Anahita Farajzadeh - Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Mohsen Mirzaee - Department of Laboratory Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Shahram Nanekarani - Department of Animal Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Reza Yari - Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
خلاصه مقاله:
Anahita Farajzadeh - Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Mohsen Mirzaee - Department of Laboratory Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Shahram Nanekarani - Department of Animal Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Reza Yari - Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Background: Acinetobacter baumannii is a common cause of nosocomial infections. A prominent feature ofthese bacteria is resistance to carbapenems. This study aimed to identify OXA genes encoding oxacillinase inAcinetobacter baumannii isolates.Methods: This cross-sectional descriptive study was performed on ۲۵ environmental A. baumannii isolatescollected from ICU over ۸ months. Definitive identification of isolates was performed by biochemical tests andpolymerase chain reaction (PCR) of ۱۶s rRNA gene. Antibiotic susceptibility testing was performed on Müller-Hinton agar medium by disk diffusion and E-test. Antibiogram and multiplex PCR data of beta-lactamase geneswere collected and analyzed at a significance level of P < ۰.۰۵ using SPSS ۲۲.۰.Results: Except for one isolate, all isolates (۹۶%) were sensitive to polymyxin B and ۸۰% of isolates weresensitive to oxacillin. All isolates were sensitive to meropenem, ampicillin/sulbactam, gentamicin, amikacin,piperacillin, and carbenicillin. The results showed that ۲۵ isolates (۱۰۰%) had OXA-۵۱ gene, ۲۱ isolates (۸۴%)had OXA-۵۸ gene, one isolate (۴%) had OXA-۲۴ gene, and none of the isolates contained OXA-۲۳ gene. Onlyisolate No.۱۰ had three oxacillinase genes simultaneously and it was resistant to oxacillin, polymyxin B, andcephalothin.Conclusions: The study showed that environmental isolates of ICU do not have pathogenic genes present in theclinical isolates, and how these genes are transferred to the peripheral isolates is an important point that shouldbe studied. Identification of genes encoding carbapenem resistance may help to understand the mechanisms ofresistance transfer in A. baumannii. The lack of the OXA-۲۳ gene plays an important role in the susceptibility ofisolates to antibiotics and non-emergence of resistant strains.
کلمات کلیدی: Acinetobacter baumannii, Carbapenems, Antibiotic resistance, Nosocomial infection
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1500635/