Seyedeh Sara Karimian - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Masoud Soleimani - Department of Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran

Differentiation of humanmesenchymal stem cells (hMSC) to neural cells on Nano-scaffolds is a promising method for the treatmentof the damaged nervous system through bionanomaterial-cell transplantation. The hMSC’s multipotential features have been discoveredin various tissue engineering researches. This investigation shows the in-vitro development and neural differentiation ofhMSC in ۳D and ۲D environments. The ۳D environment which used in this study is nanofibrous polycaprolactone (PCL). The differentiationpotential of mesenchymal stem cells (MSCs) to neural cells, on the random polycaprolactone (PCL) nanofibrous scaffolds,and tissue plate was examined. Researches have proved that interaction of extracellular nanofibrous matrix with in-vivo cells, givesmechanical maintenance to the cells and plays a functional role in the control of cellular behaviour. Stem cells are developingas a fundamental tool in the evolution of tissue engineering and regenerative medication. PCL characterization was determinedemploying scanning electron microscopy (SEM). Agents like, retinoic acid, epidermal growth factor (EGF), fibroblast growth factor(FGF-۲), and Ibmx, which they are neural inducing agents, added in DMDM/F۱۲ to differentiate MSCs to neural cells. Reproduction ofmesenchymal cells on PCL nanofibrous scaffolds and neural morphology revealed through a scanning electron microscope (SEM)and optical microscope outcomes. The differentiated mesenchymal cells on nanofibrous scaffolds express neural gene markers including; - tubulin III and Map۲ on the day of ۱۴. Our investigation recommends the potential usage of differentiated neural cellsfrom hMSCs on Nano-scaffolds toward the improvement of neural cells. This study conducted in ۲۰۱۱.

Human Mesenchymal Stem Cells, Neural Cells, Polycaprolactone Scaffolds, Tissue Engineering, Cell Therapy